乳孢素是一種從鏈霉菌中分離出的微生物代謝產(chǎn)物，現(xiàn)已廣泛用作20S蛋白酶體的選擇性抑制劑[1],[2],[3]。乳孢素首先通過(guò)其在幾種腫瘤細(xì)胞系中誘導(dǎo)分化和抑制細(xì)胞周期進(jìn)展的能力而被表征。在2到10微米的濃度下，乳孢素能夠誘導(dǎo)神經(jīng)母細(xì)胞瘤細(xì)胞系Neuro2a的神經(jīng)突起的生長(zhǎng)[4]。乳孢素不可逆地烷基化20S蛋白酶體的X亞基。蛋白酶體肽酶活性的共同抑制導(dǎo)致各種泛素化蛋白的積累，這些蛋白本應(yīng)快速降解。因此，乳孢素的效應(yīng)是多樣的，且在所處理的細(xì)胞中的信號(hào)蛋白的表達(dá)模式中具有重要的依賴性。

產(chǎn)品性質(zhì)：

Cas No.:133343-34-7

別名:蛋白酶體抑制劑,乳孢素

化學(xué)名: 2-acetamido-3-[3-hydroxy-2-(1-hydroxy-2-methylpropyl)-4-methyl-5-oxopyrrolidine-2-carbonyl]sulfanylpropanoic acid

Canonical SMILES:CC1C(C(NC1=O)(C(C(C)C)O)C(=O)SCC(C(=O)O)NC(=O)C)O

分子式:C15H24N2O7S

分子量:376.42

溶解度:1mg/mL in ethanol, 20mg/mL in DMSO, or in DMF

儲(chǔ)存條件:Store at -20°C

注意事項(xiàng)：

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References:

[1]. Omura, S., Fujimoto, T.T., Otoguro, K., et al. Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells. Journal of Antibiotics 44, 113-116 (1991).

[2]. Corey, E.J., and Reichard, G.A. Total synthesis of lactacystin. Journal of the American Chemical Society 114, 10677-10678 (1992).

[3]. Fenteany, G., and Schreiber, S.L. Lactacystin, proteasome function, and cell fate. The Journal of Biological Chemisty 273(15), 8545-8548 (1998).

[4]. Fenteany, G., Standaert, R.F., Reichard, G.A., et al. A β-lactone related to lactacystin induces neurite outgrowth in a neuroblastoma cell line and inhibits cell cycle progression in an osteosarcoma cell line. Proceedings of the National Academy of Sciences of the United States of America 91, 3358-3362 (1994).